Skip to Main content Skip to Navigation
Journal articles

Interaction of a Model Peptide on Gram Negative and Gram Positive Bacterial Sliding Clamps

Abstract : Bacterial sliding clamps control the access of DNA polymerases to the replication fork and are appealing molecular targets for antibacterial drugs development. To this end, it is critical to decipher the polymerase-clamp binding mode across various bacterial species. We showed previously that synthetic peptides targeting the clamp binding pocket of Gram-bacteria poorly interact with that of Gram+ homologous proteins. Here we analyzed the interaction of a reference peptide with several E. coli and B. subtilis clamp variants. For both Gram-and Gram+ pockets, the peptide binds through an induced-fit process but the complex stability
Keywords : sliding clamp ITC kinITC
Complete list of metadatas

Cited literature [38 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02379658
Contributor : Gilles Guichard <>
Submitted on : Monday, November 25, 2019 - 6:01:46 PM
Last modification on : Friday, November 27, 2020 - 4:34:03 PM
Long-term archiving on: : Wednesday, February 26, 2020 - 9:31:01 PM

Files

Article master 16 oct 2018_GG...
Files produced by the author(s)

Identifiers

Citation

Christophe André, Isabelle Martiel, Philippe Wolff, Marie Landolfo, Bernard Lorber, et al.. Interaction of a Model Peptide on Gram Negative and Gram Positive Bacterial Sliding Clamps. ACS Infectious Diseases, American Chemical Society, 2019, 5 (6), pp.1022-1034. ⟨10.1021/acsinfecdis.9b00089⟩. ⟨hal-02379658⟩

Share

Metrics

Record views

288

Files downloads

742