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Measurement of Biochemical Methane Potential of Heterogeneous Solid Substrates: Results of a Two- Phase French Inter-Laboratory Study

Abstract : Biochemical methane potential (BMP) is essential to determine the production of methane for various substrates; literature shows important discrepancies for the same substrates. In this paper, a harmonized BMP protocol was developed and tested with two phases of BMP tests carried out by eleven French laboratories. Surprisingly, for the three same solid tested substrates (straw; raw mix and dried-shredded mix of potatoes, maize, beef meat and straw; and mayonnaise), the standard deviations of the repeatability and reproducibility inter-laboratory were not enhanced by the harmonized protocol (average of about 25% depending on the substrate), as compared to a previous step where all laboratories used their own protocols. Moreover, statistical analyses of all the results, after removal of the outliers (about 15% of all observations), did not highlight significant effect of the operational effect on BMP (stirring, automatic or manual gas quantification, use of trace metal, uses a bicarbonate buffer, inoculum to substrate ratio) at least for the tested ranges. On the other hand, the average intra-laboratory repeatability was low, about 7%, whatever the protocol, the substrate and the laboratory. It also appears that drying the SA substrate, which contained proteins, carbohydrates, lipids and fibers, does not impact its BMP.
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Submitted on : Monday, October 12, 2020 - 8:40:36 AM
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Thierry Ribeiro, Romain Cresson, Sébastien Pommier, Sébastien Preys, Laura André, et al.. Measurement of Biochemical Methane Potential of Heterogeneous Solid Substrates: Results of a Two- Phase French Inter-Laboratory Study. Water, MDPI, 2020, 12 (10), pp.2814. ⟨10.3390/w12102814⟩. ⟨hal-02963944⟩

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