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Intégration et optimisation de procédés de séparation d'ADN

Jeffrey Teillet 1
1 LAAS-MILE - Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement
LAAS - Laboratoire d'analyse et d'architecture des systèmes
Abstract : This manuscript describes and discusses the integration of instrumentation hardware for the µLAS system for the optimisation of the analysis and separation of DNA molecules. First we will discuss separation techniques, their approaches and strategies. And we will try to answer the question that is asked when a species migrates: how to characterise the competition between forced advection and natural diffusion? In order to optimise and improve the performance of the different separation technologies thus discussed, it is necessary to introduce the problems inherent to each one. Indeed, there is a wide range of actuating means that cause molecules to migrate in many different ways: by hydrodynamics, electrophoresis or even techniques mixing both approaches. Next we will introduce µLAS technology and the technological context in which it is placed. We will present the physical principles that govern the different stages of the technology's operation (concentration and separation). We will then discuss the developments made during this thesis work on a temporal separation mode involving a new chip model. Finally, we will show the development work, both in terms of technological fabrication but also in terms of software intelligence, in order to set up slope etchings in silicon and thus enrich the µLAS system. Then we will introduce the instrumentation work carried out throughout these 3 years on a dedicated experimental bench. This chapter will be organised in such a way as to introduce an uninformed reader to the basics and problems inherent in the programming, instrumentation and automation of systems controlled by LabVIEW. We will use these basics to present the platform thus developed to drive experiments! ations µLAS. Finally, such a system needs to be characterised because it involves many players: computer and mechanical tools. Finally, we will present the separation results obtained thanks to the fully integrated and automated µLAS platform. The analysis of these results raised questions and at the same time a study of the dynamic dispersion of DNA bands. We will conclude with some perspectives for improvement, which were mentioned during the manuscript.
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Submitted on : Thursday, October 1, 2020 - 9:14:39 AM
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Jeffrey Teillet. Intégration et optimisation de procédés de séparation d'ADN. Micro et nanotechnologies/Microélectronique. Université Toulouse 3 Paul Sabatier (UT3 Paul Sabatier), 2020. Français. ⟨tel-02954397⟩

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