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Etude de la contamination fongique des environnements intérieurs par la détermination et la mesure de traceurs chimiques spécifiques : application à l'hygiène de l'habitat

Abstract : Disease occurrence in mouldy environments is very widely described in the literature. Health effects of moulds and associated metabolites are numerous: infection, allergy, toxi-infection, irritations. Prevalence and severity of declared diseases in the childs and adults seem correlated with an increase of indoor contaminated surfaces. However, the characterization of occupant exposure remains underestimated. The object of this work is essentially focused on the development of analytical methods to determine microbiological indoor quality, the definition and validation of survey indicators. This research is thus both aimed at the development and improvement of sampling techniques and also on the conception and adaptation of direct and indirect measurements methods of metabolism products ( Specific VOC) or of specific cellular components (Ergosterol, mycotoxins). The study of VOC of fungus origin enabled the definition of relevant chemical tracers associated with fungus development. >From these compounds, we established an index of fungus contamination in the indoor environments. Samplings realized in 12 houses allowed the validation of this index. Besides, an original methodology of quantification of mould exposure was proposed taking into account the evaluation of biomass and airborne mycotoxins. The transfer rate of material-air of a mycotoxin (the sterigmatocystin) was also studied.
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https://hal-cstb.archives-ouvertes.fr/tel-03840867
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Submitted on : Sunday, November 6, 2022 - 11:35:16 AM
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Stéphane Moularat. Etude de la contamination fongique des environnements intérieurs par la détermination et la mesure de traceurs chimiques spécifiques : application à l'hygiène de l'habitat. Microbiologie et Parasitologie. Université de Marne-la-Vallée, 2005. Français. ⟨NNT : ⟩. ⟨tel-03840867⟩

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